Extras: A dna Extraction sea shanty

Title: Pipette away me Tessa

To the tune of ‘Heave away me Johnny’; a traditional Newfoundland windlass shanty.

Adapted for the 'Poetry of Science' competition, 2021.

[Slower, traditional]

Oh, Tessa was a grad stu-dent,

and today extracting day.

Pipette away me Tessa, P-i-ppette away

And she was from Ho-bart,

For as long as she could stay.

Pipette away me Tessa ahoy, we're all spellbound to know.

Our hands are in their gloves now,

and de-finit-ely no thongs!

Pipette away me Tessa, P-i-ppette away

We've use a con-trol s-a-mple,

To know if we went wrong.

Pipette away me Tessa ahoy, we're all spellbound to know.

There's beetle vouchered for pin-ning down and some for genetic seq-uence, Pipette away me Tessa, P-i-ppette away And some that's bound for the Proteinase K A DNA freeing romance Pipette away me Tessa ahoy, we're all spellbound to know. The mix it is incu-bating for Two hours to provide Pipette away me Tessa, P-i-ppette away And then some buffer will be in a-gain With some ethanol also inside Pipette away me Tessa ahoy, we're all spellbound to know.

To come through spin col-umns membranous pads Who spin in cent-ri-fuge form Pippette away me Tessa, P-i-ppette away Be sure your flow through is up and gone Or no wash of chemicals borne Pippette away me Tessa ahoy, we're all spellbound to know. Farewell to all you impurity swirls Farewell my pipette tip flock Pippette away me Tessa, P-i-ppette away

It will need repeating again We'll keep supernatant stock Pippette away me Tessa ahoy, we're all spellbound to know.

DNAs bound for PCR now and some fridge bound to stay Pippette away me Tessa, P-i-ppette away And if not labelled in advance,

your samples you will mislay Pippette away me Tessa ahoy, we're all spellbound to know.

[Faster, modern] How to infer old pro-cesses Swaying species new add-resses We’ve a way, me jollies We’ve a way Come get your DNA in order Phylogenetics/geography border We’ve away, me jolly boys We've all found a way

We’ve a way, me jolly boys We've all found a way We’ve a way, me jolly boys We've all found a way